Abstract. This scientific article discusses the biological significance of enzymes, their functions in living organisms, and their role in metabolic processes. Enzymes are of great importance as biological catalysts that accelerate biochemical reactions occurring in the organism. The article provides detailed information about the structure, properties, classification, mechanism of action, and application of enzymes in medicine, the food industry, and biotechnology. In addition, scientific analyses are presented regarding the role of enzymes in maintaining human health and diseases associated with enzyme deficiency.