Abstract. In this article, the results of the research conducted to create varieties resistant to spider mite using the MAS (marker-based selection) method are highlighted. Initially, resistant and susceptible to spider mite, as well as genotypically polymorphic samples were isolated with the help of specific primers for the resistance gene to sucking pests. Then they were crossbred and hybrid combinations were obtained. Genotypes resistant to spider mite were selected from the F2 generations of hybrid combinations using specific primers and phenotypic analysis and recommended to be used as starting material in researches to create spider mite resistant varieties.